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Based on basic health research data at the end of March 2017, 23 patients were reported with positive Leptopsirosis with at least 8 deaths in Pacitan district, East Java. Leptospirosis is spread in 5 sub-districts in Pacitan Regency, namely Ngadirojo, Tulakan, Kebon Agung, Arjosari and Punung. The purpose of the study was to determine the factors that influence the control behavior of mice in the area at risk of transmission of leptospirosis. Explanatory study with survey research methods was used in this study. 92 families affected by leptospirosis were recruited through simple random sampling. The results showed that the role of health workers who are active in eradicating rats has 5.3 times the possibility of good behavior in eradicating rats when compared with the role of health workers who are less active. It can be concluded that knowledge, attitudes, information availability, and the role of health workers influence the control behavior of mice in areas at risk of leptospirosis transmission in the community.