A laboratory-based study of COVID-19 in Casablanca, Morocco
Background: Given the spread of coronavirus disease 2019 (COVID-19) and its impact on human health, laboratory confirmation of diagnosis is essential. Objective: This study examined the contribution of laboratory diagnosis to the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the diagnosis of COVID- 19, taking into account patient risk of exposure to SARS-CoV-2, clinical symptoms and comorbidities. Methods: A cross-sectional, laboratory-based study was carried out from 1 April 2020 to 30 April 2020 at the National Reference Laboratory in Morocco using nasopharyngeal samples from patients admitted to the Cheikh Khalifa International University Hospital or other hospitals in Casablanca. A one-step reverse transcription real-time polymerase chain reaction (RT-PCR) was used to detect the presence of the SARS-CoV-2 genome. A national epidemiological investigation form was used to analyze patient exposure risk, clinical symptoms and comorbidities. Results: A total of 793 samples from 375 patients were analyzed and 1150 RT-PCR tests were conducted; 116 patients (30.93%) were COVID-19 positive. Travel to a risk zone, contact with a confirmed COVID-19 case and contact with a person who had been in a risk zone were significantly associated with being positive for COVID-19. Fever and cough were the main symptoms; 7.76 % of positive patients were asymptomatic. Conclusion: This is the first laboratory-based study in Morocco for the diagnosis of COVID- 19. Laboratory diagnosis of COVID-19 by RT-PCR associated with knowledge of exposure risk factors and clinical symptoms and comorbidities remains essential for clinicians for early, appropriate medical management COVID-19 patients.
WHO. World Health Organization : COVID-19 DISEASE 2019 (COVID-19) 2020a [10 April 2020]. Available from: https://www.who.int/fr/emergencies/diseases/novel-coronavirus-2019/advice-for-public/q-a-coronaviruses.
Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, et al. A Novel Coronavirus from Patients with Pneumonia in China, 2019. The New England journal of medicine. 2020;382(8):727-33. DOI: https://doi.org/10.1056/NEJMoa2001017
WHO. World Health Organization, Coronavirus disease 2019 (COVID-19) Situation Report – 101 2020b [02 May 2020]. Available from: https://www.who.int/docs/default-source/coronaviruse/situation-reports/20200430-sitrep-101-covid-19.pdf.
Rothan HA, Byrareddy SN. The epidemiology and pathogenesis of coronavirus disease (COVID-19) outbreak. Journal of autoimmunity. 2020;109:102433. DOI: https://doi.org/10.1016/j.jaut.2020.102433
Wang M, Cao R, Zhang L, Yang X, Liu J, Xu M, et al. Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro. 2020;30(3):269-71. DOI: https://doi.org/10.1038/s41422-020-0282-0
Lu R, Zhao X, Li J, Niu P, Yang B, Wu H, et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. 2020;395(10224):565-74. DOI: https://doi.org/10.1016/S0140-6736(20)30251-8
Liu P, Shi L, Zhang W, He J, Liu C, Zhao C, et al. Prevalence and genetic diversity analysis of human coronaviruses among cross-border children. Virol J. 2017;14(1):230-. DOI: https://doi.org/10.1186/s12985-017-0896-0
Phan T. Novel coronavirus: From discovery to clinical diagnostics. Infect Genet Evol. 2020;79:104211. DOI: https://doi.org/10.1016/j.meegid.2020.104211
Guarner J. Three Emerging Coronaviruses in Two Decades. American journal of clinical pathology. 2020;153(4):420-1. DOI: https://doi.org/10.1093/ajcp/aqaa029
Wu D, Wu T, Liu Q, Yang Z. The SARS-CoV-2 outbreak: What we know. International journal of infectious diseases. 2020;94:44-8. DOI: https://doi.org/10.1016/j.ijid.2020.03.004
Konrad R, Eberle U, Dangel A, Treis B, Berger A, Bengs K, et al. Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020. Eurosurveillance. 2020;25(9):2000173. DOI: https://doi.org/10.2807/1560-7917.ES.2020.25.9.2000173
Li Z, Yi Y, Luo X, Xiong N, Liu Y, Li S, et al. Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis. 2020. DOI: https://doi.org/10.1002/jmv.25727
WHO. Recours aux méthodes de laboratoire pour le diagnostic du SRAS 2020c [10 April 2020]. Available from: https://www.who.int/csr/sars/labmethodsfr/en/.
Lai CC, Shih TP, Ko WC, Tang HJ, Hsueh PR. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and coronavirus disease-2019 (COVID-19): The epidemic and the challenges. International journal of antimicrobial agents. 2020;55(3):105924. DOI: https://doi.org/10.1016/j.ijantimicag.2020.105924
Emami A, Javanmardi F, Pirbonyeh N, Akbari A. Prevalence of Underlying Diseases in Hospitalized Patients with COVID-19: a Systematic Review and Meta-Analysis. Archives of academic emergency medicine. 2020;8(1):e35. DOI: https://doi.org/10.1371/journal.pone.0241265
Lauer SA, Grantz KH, Bi Q, Jones FK, Zheng Q, Meredith HR, et al. The Incubation Period of Coronavirus Disease 2019 (COVID-19) From Publicly Reported Confirmed Cases: Estimation and Application. Annals of internal medicine. 2020;172(9):577-82. DOI: https://doi.org/10.7326/M20-0504
Wang D, Hu B, Hu C, Zhu F, Liu X, Zhang J, et al. Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus–Infected Pneumonia in Wuhan, China. Jama. 2020;323(11):1061-9. DOI: https://doi.org/10.1001/jama.2020.1585
[The epidemiological characteristics of an outbreak of 2019 novel coronavirus diseases (COVID-19) in China]. Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi. 2020;41(2):145-51.
Bai Y, Yao L, Wei T, Tian F, Jin DY, Chen L, et al. Presumed Asymptomatic Carrier Transmission of COVID-19. Jama. 2020;323(14):1406-7. DOI: https://doi.org/10.1001/jama.2020.2565
Yu X, Yang R. COVID-19 transmission through asymptomatic carriers is a challenge to containment. 2020. DOI: https://doi.org/10.1111/irv.12743
Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020;395(10223):497-506. DOI: https://doi.org/10.1016/S0140-6736(20)30183-5
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