Mosquito vector diversity and abundance in southern Botswana, in a global context of emerging pathogen transmission


  • Ntebaleng Makate Department of Biological Sciences, University of Botswana, Gaborone
  • Pleasure Ramatlho Department of Biological Sciences, University of Botswana, Gaborone
  • Tefo Kesaobaka Kgoroebutswe Department of Biological Sciences, University of Botswana, Gaborone
  • Katherine Laycock Children's Hospital of Philadelphia, Philadelphia, Pennsylvania
  • Giacomo Maria Paganotti Botswana-University Pennsylvania Partnership, Gaborone, Botswana; Division of Infectious Diseases, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Biomedical Sciences, University of Botswana, Gaborone



Aedes aegypti, Culex spp, surveillance, ovitrap


Background. The continued spread of infectious diseases by mosquitoes remains a formidable obstacle to the well-being of the people all over the world. Arboviruses are spread from one vertebrate host to another by vectors through intricate transmission cycles that involve the virus, the vertebrate host, and the vector. It is essential to acquire a better understanding of the current abundance and distribution of major vectors in order to adequately prepare for the possibility of arbovirus outbreaks. This is because the abundance and distribution of these major vectors determines the human populations that are at risk for the diseases that they transmit. The effects of climate change on the amount of mosquitoes and their ability to survive the seasons have had a substantial impact on the spread of diseases that are transmitted by vectors in many different parts of Botswana.
Methods. The purpose was to collect mosquito samples in Gaborone and the neighboring areas in southern Botswana, including border stations. We collected different stages of the mosquito from each place, raised them to maturity, and then identified them. Both morphological and genetic studies were utilized in order to successfully identify the organism. The species of Culex mosquitoes accounted for 88.3% of the 5177 mosquitoes that were collected and identified, whereas the species of Aedes aegypti and Anopheles mosquitoes accounted for 11.5% and 0.2% respectively.
Conclusions. These findings give entomological baseline data that will aid in the study of vectorial patterns and the estimation of future arboviral hazards provided by mosquitoes. Additionally, these findings document the diversity and abundance of mosquito species.

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Lee H, Halverson S, Ezinwa, N. Mosquito-Borne Diseases. Primary Care-Clinics in Office Practice 2018; 45(3), 393–407. DOI:

Tolle MA, Mosquito-borne Diseases. Current Problems in Paediatric and Adolescent Health

Care 2009; 39(4), 97–140. DOI:

Hill SC, Vasconcelos J, Neto Z, et al. Emergence of the Asian lineage of Zika virus in Angola: an outbreak investigation. The Lancet Infectious Diseases 2019; 19 1138–1147. DOI:

Musso D. Gubler DJ. Zika virus. Clinical Microbiology Reviews 2016; 29(3), 313–320. DOI:

Vorou R. Zika virus, vectors, reservoirs, amplifying hosts, and their potential to spread worldwide: What we know and what we should investigate urgently. International Journal of Infectious Diseases 2016; 48, 85–90. DOI:

Chirebvu E. Chimbari MJ. Characteristics of Anopheles arabiensis larval habitats in Tubu village, Botswana Journal of Vector Ecology 2014; 40(1), 129–138. DOI:

Pachka H, Annelise T, Alan K et al. Rift Valley fever vector diversity and impact of meteorological and environmental factors on Culex pipiens dynamics in the Okavango Delta, Botswana. Parasites & Vectors 2016; 9:434. DOI:

Tawe L, Ramatlho P, Waniwa K. et al. Preliminary survey on Anopheles species distribution in Botswana shows the presence of Anopheles gambiae and Anopheles funestus complexes. Malaria Journal 2017; 16:106. DOI:

Bango ZA, Tawe L, Waithaka C, Paganotti GM. Past and current biological factors affecting malaria in the low transmission setting of Botswana : A review. Infection, Genetics and Evolution 2020; 85:104458. DOI:

Simon C, Moakofhi K, Mosweunyane T. et al. Malaria control in Botswana, 2008-2012: the path towards elimination. Malaria Journal 2013; 12:458. DOI:

Kgoroebutswe TK, Ramatlho P, Reeder S. et al. Distribution of Anopheles mosquito species, their vectoral role and profiling of knock-down resistance mutations in Botswana. Parasitology Research 2020; 119, 1201–1208. DOI:

Rueda LM, Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with Dengue Virus Transmission 2004 Auckland, New Zealand: Mongolia Press. DOI:

Scott JA, Collins FH & Brogdon WG. Identification of Single Specimens of the Anopheles gambiae Complex by the Polymerase Chain Reaction. The American Journal of Tropical Medicine and Hygiene 1993; 49(4), 520–529. DOI:

Cornel AJ, Lee Y, Paulo A, et al. Mosquito community composition in South Africa and some neighbouring countries. Parasites & Vectors 2018; 11:331. DOI:

Jupp P. Mosquitoes of Southern Africa: Culicinae and Toxorhynchitinae. Hartebeespoort: Ekogilde Publishers 1996.

Braack L, Gouveia De Almeida AP, Cornel AJ. et al. Mosquito-borne arboviruses of African origin: Review of key viruses and vectors. Parasites and Vectors 2018; 11:29. DOI:

Chouin-Carneiro T, Vega-Rua A, Vazeille M. et al. Differential Susceptibilities of Aedes aegypti and Aedes albopictus from the Americas to Zika Virus. PLoS Neglected Tropical Diseases 2016; 10(3), e0004543. DOI:

Weetman D, Kamgang B, Badolo A et al. Aedes mosquitoes and Aedes-borne arboviruses in Africa: Current and future threats. International Journal of Environmental Research and Public Health 2018; 15(2), 220. DOI:

Mweya CN, Kimera SI, Stanley G. et al. Climate change influences potential distribution of infected Aedes aegypti co-occurrence with dengue epidemics risk areas in Tanzania. PLoS ONE 2016; 11(9), DOI:

Yalwala S, Clark J, Oullo D. et al. Comparative efficacy of existing surveillance tools for Aedes aegypti in Western Kenya. Journal of Vector Ecology 2015; 40(2), 301–307. DOI:

Mier-y-Teran-Romero L, Tatem AJ, Johansson MA. Mosquitoes on a plane: Disinfection will not stop the spread of vector-borne pathogens, a simulation study. PLoS Neglected Tropical Diseases 2017; 11(7), e0005683. DOI:

Buxton M, Lebani K, Nyamukondiwa C, Wasserman RJ. First record of Aedes (Stegomyia) aegypti (Linnaeus, 1762) (Diptera: Culicidae) in Botswana. BioInvasions Records 2019; 8(3), 551–557. DOI:

Freedman O. The yellow fever situation in the Bechuanaland protectorate. In World Health Organization 1954; Geneva, Switzerland.

Muspratt J. The Stegomyia mosquitoes of South Africa and some neighbouring territories. Memoirs of the Entomological Society of Southern Africa 1956; 4, 1–138.

Murrell EG, Damal K, Lounibos LP, Juliano SA. Distributions of competing container mosquitoes depend on detritus types, nutrient ratios, and food availability. Annals of the Entomological Society of America 2011; 104(4), 688–698. DOI:

Juliano SA, O’Meara GF, Morrill JR & Cutwa MM. Desiccation and thermal tolerance of eggs and the coexistence of competing mosquitoes. Oecologia 2002; 130(3), 458–469. DOI:

BOPA. Ministry cautions public on malaria outbreak. Retrieved from Daily News website: 2018.

Kline DL. Traps and trapping techniques for adult mosquito control. Journal of the American Mosquito Control Association 2006; 22(3), 490–496.[490:TATTFA]2.0.CO;22125 DOI:[490:TATTFA]2.0.CO;2

Silver JB. Mosquito Ecology: Field Sampling Methods. Retrieved from 2008.




How to Cite

Makate, N., Ramatlho, P., Kgoroebutswe, T. K., Laycock, K., & Paganotti, G. M. (2022). Mosquito vector diversity and abundance in southern Botswana, in a global context of emerging pathogen transmission. Journal of Public Health in Africa, 13(3).



Original Articles