Antiosteoarthritis activities of 70% ethanol extract of <em>Eleutherine bulbosa</em> (mill.) urb. bulb on rats monosodium iodoacetate-induced osteoarthritis

Riza Ambar Sari; Fina Luthfiana; Irawati Sholihah; Katsuyoshi Matsunami; Sukardiman Sukardiman; Retno Widyowati

doi:10.4081/jphia.2023.2506

Authors

Riza Ambar Sari Master Program in Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia.
Fina Luthfiana Master Program in Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia.
Irawati Sholihah Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.
Katsuyoshi Matsunami Department of Pharmacognosy, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Sukardiman Sukardiman Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya; Natural Products Drug Discovery and Development, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia.
Retno Widyowati
rr-retno-w@ff.unair.ac.id
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya; Natural Products Drug Discovery and Development, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia.

Background: Osteoarthritis (OA) is a common degenerative joint situation that induces pain and disability in the elderly. Traditionally, Eleutherine bulbosa bulb from Pasuruan, East Java, is used to treat many diseases, also as an anti-inflammatory.

Objective: In this research, we employed an in vivo model to examine the effects of 70% ethanol extracts of E. bulbosa (EBE) on the progression and development of OA.

Methods: A singular intraarticular injection of Monosodium Iodoacetate (MIA) was used to create the OA model in rats. The progression of OA was observed for three weeks. Furthermore, treatment of EBE at a dose of 6, 12, and 24 mg/200g BW orally for four weeks was conducted to assess the effects on decreasing IL- 1ß level, joint swelling, and hyperalgesia.

Results: Induction was successful, indicated by a significant difference (P<0.05) in decreasing latency time, increasing joint swelling, and IL-1ß level. EBE 24 mg/200 g BW treatment has significantly (P<0.05) reduced IL-1ß levels, joint swelling, and response to hyperalgesia.

Conclusion: The 70% ethanol extract of E. bulbosa bulb has therapeutic effects on inflammation through reducing IL-1ß in experimental MIA-induced osteoarthritis in a rat model. According to this study, EBE may have an effective potential new agent for OA therapy.

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Sari, R. A., Luthfiana, F., Sholihah, I., Matsunami, K., Sukardiman, S., & Widyowati, R. (2023). Antiosteoarthritis activities of 70% ethanol extract of <em>Eleutherine bulbosa</em> (mill.) urb. bulb on rats monosodium iodoacetate-induced osteoarthritis. Journal of Public Health in Africa, 14(s1). https://doi.org/10.4081/jphia.2023.2506