Effects of Eleutherine bulbosa (mill.) urb. bulb extract on mice glucocorticoid-induced osteoporosis models

Published: 16 March 2023
Abstract Views: 118
PDF: 102
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.


  • Fina Luthfiana Master Program of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Indonesia.
  • Riza Ambar Sari Master Program of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Indonesia.
  • Irawati Sholikhah Department of Chemistry, Faculty of Sains and Technology, Universitas Airlangga, Indonesia.
  • Katsuyoshi Matsunami Department of Pharmacognosy, Graduate School of Biomedical & Health Sciences, Hiroshima University, Japan.
  • Sukardiman Sukardiman Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga; Natural Products Drug Discovery and Development, Faculty of Pharmacy, Universitas Airlangga, Indonesia.
  • Retno Widyowati Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga; Natural Products Drug Discovery and Development, Faculty of Pharmacy, Universitas Airlangga, Indonesia.

Background: Low bone mass accompanied by microarchitectural alterations in the bone that cause fragility fractures is known as secondary osteoporosis and occurs when there is an underlying condition or medication present. Eleutherine bulbosa bulb extract has been shown to affect bone because of its content, which can help osteoblast differentiation and inhibit osteoclast differentiation.

Objective: This study aimed to assess the effects of 70% ethanol extract of E. bulbosa Bulbs (EBE) from Pasuruan-East Java on blood calcium levels, osteoblast cell count, and bone density of trabecular femur in osteoporosis rats.

Methods: Six groups of 30 female Wistar rats were created. There were no test materials offered to the healthy group; the negative group received 0.5% CMC; the positive group received alendronate 0.9 mg/kg BW; and the dose group received 30, 60, and 120 mg/kg BW. Glucocorticoid (Dexamethasone) 0.1015 mg/kg BW/day induction was given to all groups except the healthy group to create osteoporosis rats for approximately four weeks. Then they were given oral therapy for approximately 28 days. Followed by the determination of blood calcium levels, the number of osteoblast cells, and bone density of the rat femur trabecular.

Results: The result showed that E. bulbosa bulbs extract could raise blood calcium levels and bone density percentage at doses of 60 and 120 mg/kg BW, as well as raise osteoblast cell levels at doses of 120 mg/kg BW.

Conclusions: The findings indicate that E.bulbosa bulb extract is a potential complementary medicine for osteoporosis.

Laven JS, Visser JA, Uitterlinden AG, et al. Menopause: genome stability as new paradigm. Maturitas 2016;92:15-23. DOI: https://doi.org/10.1016/j.maturitas.2016.07.006

Bone Health and Osteoporosis Foundation (BHOF). Osteoporosis fast facts. 251 18th Street S, Suite 630, Arlington, VA, 22202.

Cohen JE, Wakefield CE, Cohn RJ. Nutritional interventions for survivors of childhood cancer. Cochrane Database Syst Rev 2016;CD009678. DOI: https://doi.org/10.1002/14651858.CD009678.pub2

Paccou J, Edwards MH, Ward K, et al. Relationships between bone geometry, volumetric bone mineral density and bone microarchitecture of the distal radius and tibia with alcohol consumption. Bone 2015;78:122-9. DOI: https://doi.org/10.1016/j.bone.2015.05.002

Kline J, Tang A, Levin B. Smoking, alcohol and caffeine in relation to two hormonal indicators of ovarian age during the reproductive years. Maturitas 2016;92:115-22. DOI: https://doi.org/10.1016/j.maturitas.2016.07.010

Pouresmaeili F, Kamalidehghan B, Kamarehei M, Goh YM. A comprehensive overview on osteoporosis and its risk factors. Therapeut Clin Risk Manag 2018;14:2029-49. DOI: https://doi.org/10.2147/TCRM.S138000

Buehring B, Viswanathan R, Binkley N, Busse W. Glucocorticoid-induced osteoporosis: an update on effects and management. J Allergy Clin Immunol 2013;132:1019-30. DOI: https://doi.org/10.1016/j.jaci.2013.08.040

Wishart J, Horowitz M, Need A, Nordin BE. Relationship between forearm and vertebral mineral density in postmenopausal women with primary hyperparathyroidism. Arch Intern Med 1990;150:1329-31. DOI: https://doi.org/10.1001/archinte.150.6.1329

Cooper C, Coupland C, Mitchell M. Rheumatoid arthritis, corticosteroid therapy and hip fracture. Ann Rheum Dis 1995;54:49-52. DOI: https://doi.org/10.1136/ard.54.1.49

Isidro ML, Ruano B. Bone disease in diabetes. Curr Diabetes Rev 2010;6:144-55. DOI: https://doi.org/10.2174/157339910791162970

Haasum Y, Fastbom J, Fratiglioni L, Johnell, K. Undertreatment of osteoporosis in persons with dementia? A population-based study. Osteoporos Int 2012;23:1061-8. DOI: https://doi.org/10.1007/s00198-011-1636-8

Coleman RE. Clinical features of metastatic bone disease and risk of skeletal morbidity. Clin Cancer Res 2006;12:6243s-9s. DOI: https://doi.org/10.1158/1078-0432.CCR-06-0931

Licata A. Osteoporosis in men: suspect secondary disease first. Cleveland Clin J Med 2003;70:247-54. DOI: https://doi.org/10.3949/ccjm.70.3.247

Lin S, Huang J, Zheng L, et al. Glucocorticoid-induced osteoporosis in growing rats. Calsif Tissue Int 2014;95:362-73. DOI: https://doi.org/10.1007/s00223-014-9899-7

Katzung BG. Basic and clinical pharmacology 12th edition volume 1. San Fransisco: Mc Graw Hill Lange 2012.

Cohen D, Adachi JD. The treatment of glucocorticoid-induced osteoporosis. J Steroid Biochem Molecul Biol 2004;88:337-49. DOI: https://doi.org/10.1016/j.jsbmb.2004.01.003

Noor Z. Buku ajar: Osteoporosis patofisiologi dan peran atom mineral dalam manajemen terapi. Jakarta: Salemba Medika; 2014.

Agil M, Ma’arif B, Aemi NY. Antiosteoporotic activity of n-hexane fraction from Marcilea crenata Presl. leaves in increasing trabecular vertebrae bone density of female mice. J Tumbuhan Obat Indonesia 2018;11:1-7. DOI: https://doi.org/10.22435/jtoi.v11i2.671

Laswati H, Agil M, Widyowati R. Effect of Spilanthes acmella and exercise on osteoblast cells femur in mice dexamethasone induced. Media Penelitian dan Pengembangan Kesehatan 2015;25:43-50. DOI: https://doi.org/10.22435/mpk.v25i1.4095.43-50

Amelia T, Pratiwi D, Tjahjono D. In silico study of the component of Eleutherine americana MERR. on human estrogen reseptor alpha as potential anti-breast cancer. Int Conf Comput Sci Technol 2014;3:6-9. DOI: https://doi.org/10.2991/iccst-15.2015.2

Duke JA, Godwin MJB, Ottesen AR. Duke’s handbook of medicinal plants of Latin America. London: CRC Press 2008. DOI: https://doi.org/10.1201/9781420043174

Bianchi C, Ceriotti G. Chemical and pharmacological investigations of constituents of Eleutherine bulbosa (Miller) Urb. (Iridaceae). J Pharmaceut Sci 1975;64:1305-8. DOI: https://doi.org/10.1002/jps.2600640809

Hand PTB, Thao DT, Nga NT, et al. Toxicity and anti-inflammatory activities of an extract of the Eleutherine bulbosa rhizome on collagen antibody-induced arthritis in a mouse model. Natural Product Communications. 2018;13:883-6. DOI: https://doi.org/10.1177/1934578X1801300725

Uchino K, Okamoto K, Sakai E, et al. Dual effects of liquiritigenin on the proliferation of bone cells: promotion of osteoblast differentiation and inhibition of osteoclast differentiation. Phytotherapy Research. 2015;29:1714-21. DOI: https://doi.org/10.1002/ptr.5416

Weitzmann MN, Pacifici R. Estrogen deficiency and bone loss: an inflammatory tale. J Clin Invest 2006;116:1186-94. DOI: https://doi.org/10.1172/JCI28550

Takahashi M, Ushijima K, Hayashi Y, et al. Dosing-time dependent effect of dexamethasone on bone density in rats. Life Sci 2009:86;24-9. DOI: https://doi.org/10.1016/j.lfs.2009.10.020

Mirza F, Canalis E. Secondary osteoporosis: pathophysiology and management. Eur J Endocrinal 2015;173:R131-R51. DOI: https://doi.org/10.1530/EJE-15-0118

Saleem AN, Chen YH, Baek HJ, et al. Mice with alopecia, osteoporosis, and systematic amyloidosis due to mutation in Zdhhc13, a gene coding for palmitoyl acyltransferase. PLoS Genet 2010;6:e1000985. DOI: https://doi.org/10.1371/journal.pgen.1000985

Bahtiar A, Dewi R. Antiosteoporosis effects of 70% ethanolic extract combination of dayak onion bulbs (Eleutherine bulbosa (Mill.) Urb) and cowpea (Vigna unguiculata (L.) Walp.) on the hypoestrogen rats. Pharmacogn J 2019;11:632-8. DOI: https://doi.org/10.5530/pj.2019.11.101

Choi EM. Liquiritigenin isolated from Glycyrrhiza uralensis stimulates osteoblast function in osteoblastic MC3T3-E1c. International Immunopharmacology 2012;12:139-43. DOI: https://doi.org/10.1016/j.intimp.2011.11.003

Carnovali M, Luzi L, Terruzzi I, et al. Liquiritigenin reduces blood glucose level and bone adverse effects in hyperglycemic adult zebrafish. Nutrients 2019;11:1042. DOI: https://doi.org/10.3390/nu11051042

Choi EM, Suh KS, Lee YS. Liquiritigenin restores osteoblast damage through regulating oxidative stress and mitochondrial dysfunction. Phytother Res 2014;28:880-6. DOI: https://doi.org/10.1002/ptr.5071

Sun JY, Zhang H, Zhang Y, et al. Impact of serum calcium levels on total body bone mineral density: a mendelian randomization study in five age strata. Clin Nutr 2021;40:2726-33. DOI: https://doi.org/10.1016/j.clnu.2021.03.012

Dalemo S, Eggertsen R, Hjerpe P, et al. Bone minral density in primary care patients related to serum calcium concentration: a longitudinal cohort study from Sweden. Scand J Primary Health Care 2018;36:198-206. DOI: https://doi.org/10.1080/02813432.2018.1459430

Luthfiana, F., Sari, R. A., Sholikhah, I., Matsunami, K., Sukardiman, S., & Widyowati, R. (2023). Effects of <em>Eleutherine bulbosa</em> (mill.) urb. bulb extract on mice glucocorticoid-induced osteoporosis models. Journal of Public Health in Africa, 14(s1). https://doi.org/10.4081/jphia.2023.2507


Download data is not yet available.